
Figure 1: Flow chart
Variable |
N |
Mean |
Standard Deviation |
Minimum |
Maximum |
Lower Quartile |
Median |
Upper Quartile |
Cal1 |
163 |
44.5 |
75.1 |
3 |
587 |
15 |
20 |
37 |
Cal2 |
163 |
60.3 |
183.4 |
6 |
2142 |
15 |
22 |
43 |
Cal3 |
163 |
41 |
73.7 |
9 |
699 |
15 |
18 |
40 |
Cal 1-3: Calprotectin values from the first, second and third stool sample respectively. All calprotectin values are in μg/g.
Table 1: Key figures on all three faecal calprotectin measurements
|
|
|
First series |
Second series |
|
||||||
V |
Age |
Gender |
NEV |
Cal1 |
Cal2 |
Cal3 |
NEV |
Cal1 |
Cal2 |
Cal3 |
Investigation |
1 |
32 |
F |
1 |
41 |
70 |
29 |
|
|
|
|
none |
2 |
34 |
F |
1 |
23 |
19 |
68 |
|
|
|
|
none |
3 |
54 |
M |
1 |
30 |
69 |
36 |
|
|
|
|
none |
4 |
32 |
F |
1 |
21 |
15 |
69 |
|
|
|
|
none |
5 |
26 |
M |
1 |
15 |
15 |
58 |
|
|
|
|
none |
6 |
57 |
F |
1 |
21 |
41 |
52 |
|
|
|
|
none |
7 |
54 |
F |
1 |
15 |
71 |
15 |
|
|
|
|
none |
8 |
23 |
M |
1 |
28 |
47 |
88 |
|
|
|
|
none |
9 |
28 |
F |
1 |
26 |
22 |
53 |
|
|
|
|
none |
10 |
25 |
F |
1 |
47 |
51 |
39 |
|
|
|
|
none |
11 |
63 |
F |
1 |
118 |
15 |
15 |
|
|
|
|
none |
12 |
28 |
M |
1 |
15 |
49 |
132 |
|
|
|
|
none |
13 |
26 |
M |
1 |
15 |
347 |
16 |
|
|
|
|
none |
14 |
50 |
M |
2 |
31 |
75 |
55 |
|
|
|
|
none |
15 |
31 |
F |
2 |
61 |
48 |
72 |
|
|
|
|
none |
16 |
44 |
M |
2 |
90 |
44 |
51 |
|
|
|
|
none |
17 |
44 |
M |
2 |
15 |
73 |
59 |
|
|
|
|
none |
18 |
35 |
F |
2 |
37 |
305 |
94 |
|
|
|
|
gastro/colo |
19 |
52 |
F |
2 |
99 |
50 |
485 |
|
|
|
|
gastro/colo |
20 |
32 |
F |
3 |
142 |
147 |
128 |
|
|
|
|
gastro/colo |
21 |
58 |
F |
3 |
489 |
241 |
361 |
|
|
|
|
gastro/colo† |
22 |
29 |
F |
1 |
21 |
57 |
30 |
0 |
37 |
18 |
15 |
none |
23 |
51 |
F |
1 |
60 |
45 |
18 |
0 |
19 |
17 |
19 |
none |
24 |
51 |
F |
1 |
29 |
32 |
74 |
0 |
15 |
29 |
15 |
none |
25 |
41 |
M |
1 |
80 |
40 |
30 |
0 |
25 |
25 |
48 |
none |
26 |
25 |
M |
1 |
96 |
40 |
15 |
0 |
15 |
15 |
15 |
none |
27 |
45 |
F |
1 |
15 |
102 |
15 |
0 |
15 |
15 |
15 |
none |
28 |
51 |
M |
1 |
16 |
24 |
109 |
0 |
27 |
45 |
34 |
none |
29 |
44 |
F |
1 |
325 |
24 |
15 |
0 |
18 |
15 |
15 |
none |
30 |
31 |
M |
1 |
265 |
21 |
15 |
1 |
19 |
115 |
15 |
none |
31 |
21 |
F |
1 |
19 |
2142 |
15 |
1 |
15 |
15 |
60 |
none |
32 |
23 |
M |
1 |
38 |
728 |
41 |
1 |
53 |
31 |
48 |
none |
33 |
49 |
M |
1 |
45 |
59 |
31 |
2 |
53 |
15 |
254 |
none |
34 |
58 |
F |
2 |
96 |
59 |
32 |
0 |
15 |
28 |
15 |
none |
35 |
22 |
F |
2 |
16 |
107 |
91 |
0 |
15 |
15 |
15 |
none |
36 |
24 |
F |
2 |
128 |
74 |
15 |
0 |
20 |
15 |
15 |
none |
37 |
28 |
F |
2 |
151 |
192 |
36 |
1 |
75 |
30 |
23 |
none |
38 |
23 |
F |
2 |
78 |
32 |
68 |
3 |
141 |
84 |
65 |
none |
39 |
26 |
F |
2 |
193 |
25 |
124 |
3 |
122 |
166 |
110 |
gastro/colo |
40 |
17 |
M |
2 |
587 |
222 |
43 |
3 |
62 |
156 |
94 |
gastro/colo |
41 |
45 |
M |
2 |
60 |
107 |
40 |
3 |
567 |
64 |
262 |
gastro/colo |
42 |
34 |
F |
2 |
16 |
53 |
128 |
3 |
91 |
153 |
76 |
gastro/colo |
43 |
21 |
F |
3 |
134 |
266 |
71 |
0 |
15 |
20 |
15 |
none |
44 |
36 |
F |
3 |
79 |
53 |
81 |
2 |
58 |
36 |
67 |
none |
45 |
52 |
M |
3 |
57 |
349 |
51 |
1 |
81 |
15 |
15 |
none |
46 |
53 |
F |
3 |
152 |
245 |
71 |
1 |
70 |
39 |
24 |
gastro/colo† |
47 |
30 |
M |
3 |
197 |
179 |
223 |
3 |
211 |
201 |
567 |
none |
48 |
29 |
F |
3 |
140 |
297 |
699 |
3 |
805 |
687 |
716 |
gastro/colo† |
49 |
26 |
F |
3 |
343 |
89 |
66 |
3 |
1591 |
1085 |
324 |
gastro/colo |
Abbreviations: V: volunteer; NEV: number of elevated values; M: male, F: female; Cal: calprotectin; INV: investigation; gastro/colo:
gastroscopy and colonoscopy ;†H. pylori infection. All calprotectin values are inμ g/g.
Table 2: Overview of the faecal calprotectin values in the participants with elevated values, including values of the second series of three stool
samples
Elevated values |
Cut-off 50µg/g n (%) |
Cut-off 100µg/g n (%) |
Cut-off 150µg/g n (%) |
0 |
114 (69.9) |
137 (84) |
146 (89.6) |
>0 |
49 (30.1) |
26 (16) |
17 (10.4) |
- 1 |
- 25 (15.3) |
- 17 (10.4) |
- 11 (6.7) |
- 2 |
- 15 (9.2) |
- 5 (3.1) |
- 4 (2.5) |
- 3 |
- 9 (5.5) |
- 4 (2.5) |
- 2 (1.2) |
Total |
163 (100) |
163 (100) |
163 (100) |
Table 3: Faecal Calprotectin values based on cut-off
Cal |
Female (%) |
Male (%) |
Total (%) |
<50 |
69 (69) |
45 (71.4) |
114 (69.9) |
<100 |
83 (83) |
54 (85.7) |
137 (84.1) |
<150 |
89 (89) |
57 (90.5) |
146 (89.6) |
Total |
100 (100) |
63 (100) |
163 (100) |
Abbreviations: Cal: calprotectin cut-off (μg/g).
Table 4: Faecal calprotectin results based on self-reported gender
Variable |
N |
Mean |
SD |
Min |
Q1 |
Median |
Q3 |
Max |
mad |
163 |
20.168 |
64.36 |
0 |
0.33 |
4 |
13 |
709 |
SD |
163 |
33.098 |
109.74 |
0 |
0.58 |
6.93 |
21 |
1226.87 |
mean |
163 |
48.603 |
78.80 |
10.33 |
15.33 |
25.33 |
44 |
725.33 |
range |
163 |
60.503 |
193.07 |
0 |
1 |
12 |
39 |
2127 |
Abbreviations: Mad: mean of absolute deviation; SD: standard deviation; Q1/3: Quartile 1/3; Min: minimum, Max: maximum.
Supplementary Table 1: Comparison of variations
Spearman Correlation Coefficients, N = 163 Prob > |r| H0: Rho=0 |
|||||
|
mean |
med |
mad |
sd |
range |
mean |
1 |
0.87066 |
0.87621 |
0.87257 |
0.87621 |
|
<.0001 |
<.0001 |
<.0001 |
<.0001 |
|
med |
0.87066 |
1 |
0.66034 |
0.64891 |
0.66034 |
<.0001 |
|
<.0001 |
<.0001 |
<.0001 |
|
mad |
0.87621 |
0.66034 |
1 |
0.99941 |
1 |
<.0001 |
<.0001 |
|
<.0001 |
<.0001 |
|
sd |
0.87257 |
0.64891 |
0.99941 |
1 |
0.99941 |
<.0001 |
<.0001 |
<.0001 |
|
<.0001 |
|
range |
0.87621 |
0.66034 |
1 |
0.99941 |
1 |
<.0001 |
<.0001 |
<.0001 |
<.0001 |
|
Abbreviations: med: median; mad: median of absolute deviation; sd: standard deviation
Supplementary Table 2: Correlations
Figure 1: Flow chart
Abbreviations: Cal: faecal calprotectin (FC) in μg/g; cal1-3: results of the first through third FC measurement. Rep: red represents the second,
blue the third measurement.
How to read the table: the x-axis shows the first calprotectin measurement (Cal1), red on the y-axis shows the second measurement (cal2),
blue on the y-axis the third (cal3). 6 values (from 6 different volunteers) > 400 μg/g were not documented for better visibility: 587 and 489 in
the first measurement, 2142 and 728 in the second, and 699 and 485 in the third measurement.
Figure 2: Faecal Calprotectin measurements per volunteer
Feacal calprotectin in μg/g. The box plots show median and interquartile range, mean (small square) and standard deviation, as well as
outliers. 6 values (from 6 volunteers) > 400 μg/g were not documented for better visibility: 587 and 489 μg/g in the first measurement, 2142
and 728 μg/g in the second, and 699 and 485 μg/g in the third measurement.
Figure 3: Boxplots of the first (a), second (b) and third (c) calprotectin measurement
Figure of calprotectin values (μg/g) of 163 volunteers; sd: standard deviation
Supplementary Figure 1: Range comparison
Tables at a glance
Figures at a glance