Figure 1: Representative images of mycobacteriophage D29 PFU on a solid nutrient medium with M.smegmatis after incubation with animal plasma after a 20-day administration of the liposomal bacteriophage preparation.
Dilution of mycobacteriophage |
Mycobacteriophage stock in buffer solution, PFU/ml |
Mycobacteriophage after incubation with experimental plasma, PFU/ml |
Mycobacteriophage after incubation with control plasma, PFU/ml |
7 |
13 |
10 |
19 |
6 |
<150 |
<100 |
<200 |
Table 1: Determination of the bacteriophage titer by a microbiological analysis
Sample/Dilution |
1:10 |
1:100 |
1:1 000 |
1: 10 000 |
Mycobacteriophage in buffer solution |
31,78 |
35,38 |
36,61 |
37,86 0,01 |
Mycobacteriophage with plasma of control group mice |
32,08 |
34,66 |
36,99 |
Mycobacteriophage DNA was not detected (threshold cycle greater than 40) |
Mycobacteriophage with plasma of |
32,40 |
34,27 |
37,82 |
|
mice of the immune group |
0,06 |
0,02 |
0,98 |
|
Table 2: Threshold PCR-R? cycles for the determination of bacteriophage D29 DNA, average (n=2) standard deviation
Plasma dilution/test samples |
Plasma of immune mice |
Plasma from control group mice |
||||
|
1 repetition |
2 repetition |
average value |
1 repetition |
2 repetition |
average value |
1:1 |
0,635 |
0,680 |
0,658 |
0,512 |
0,546 |
0,529 |
1:2 |
0,610 |
0,588 |
0,599 |
0,472 |
0,468 |
0,470 |
1:4 |
0,560 |
0,583 |
0,572 |
0,453 |
0,497 |
0,475 |
1:16 |
0,514 |
0,490 |
0,502 |
0,410 |
0,405 |
0,408 |
1:32 |
0,392 |
0,392 |
0,392 |
0,389 |
0,399 |
0,394 |
1:64 |
0,190 |
0,191 |
0,191 |
0,155 |
0,177 |
0,166 |
1:128 |
0,031 |
0,036 |
0,034 |
0,012 |
0,014 |
0,013 |
Table 3: Antibody determination. Optical density of the studied samples in two replicates by ELISA
Figure 1: Representative images of mycobacteriophage D29 PFU on a solid nutrient medium with M.smegmatis after incubation with animal plasma after a 20-day administration of the liposomal bacteriophage preparation.
Tables at a glance
Figures at a glance