Tables at a glance

Table 1

Table 2

Table 3

Figures at a glance

Figure 1

Table 1

Dilution of mycobacteriophage

Mycobacteriophage stock in buffer solution, PFU/ml

Mycobacteriophage after incubation with experimental plasma, PFU/ml

Mycobacteriophage after incubation with control plasma, PFU/ml

7
1:10

13

10

19

6
1:10

<150

<100

<200

Table 1: Determination of the bacteriophage titer by a microbiological analysis

Table 2

Sample/Dilution	1:10	1:100	1:1 000	1: 10 000
Mycobacteriophage in buffer solution	31,78 0,08	35,38 0,24	36,61 0,54	37,86 0,01
Mycobacteriophage with plasma of control group mice	32,08 0,09	34,66 0,58	36,99 0,06	Mycobacteriophage DNA was not detected (threshold cycle greater than 40)
Mycobacteriophage with plasma of	32,40	34,27	37,82
mice of the immune group	0,06	0,02	0,98

Table 2: Threshold PCR-R? cycles for the determination of bacteriophage D29 DNA, average (n=2) standard deviation

Table 3

Plasma dilution/test samples	Plasma of immune mice			Plasma from control group mice
	1 repetition	2 repetition	average value	1 repetition	2 repetition	average value
1:1	0,635	0,680	0,658	0,512	0,546	0,529
1:2	0,610	0,588	0,599	0,472	0,468	0,470
1:4	0,560	0,583	0,572	0,453	0,497	0,475
1:16	0,514	0,490	0,502	0,410	0,405	0,408
1:32	0,392	0,392	0,392	0,389	0,399	0,394
1:64	0,190	0,191	0,191	0,155	0,177	0,166
1:128	0,031	0,036	0,034	0,012	0,014	0,013

Table 3: Antibody determination. Optical density of the studied samples in two replicates by ELISA

FIGURE 1

Figure 1: Representative images of mycobacteriophage D29 PFU on a solid nutrient medium with M.smegmatis after incubation with animal plasma after a 20-day administration of the liposomal bacteriophage preparation.