Table 1: Reagents of PCR amplification (25 ul) for MAOA gene
Table 2
Stage No.
steps
Temperature
Time
No. of cycles
1
Initial denaturation
95 ˚C
5 min
1
2
Denaturation
94 ˚C
30 sec
30
Annealing
60 ˚C
30 sec
Extension
72 ˚C
30 sec
3
Final extension
72 ˚C
5 min
1
Table 2: The program used in PCR amplification of MAOA gene
Table 3
Stage No.
steps
Temperature
Time
No. of cycles
1
Initial denaturation
95 ˚C
5 min
1
2
Denaturation
94 ˚C
30 sec
30
Annealing
60 ˚C
30 sec
Extension
72 ˚C
30 sec
3
Final extension
72 ˚C
5 min
1
Table 3: COMT rs 4680 PCR condition
Table 4
Stage No.
steps
Temperature
Time
No. of cycles
1
Initial denaturation
95 ˚C
5 min
1
2
Denaturation
94 ˚C
30 sec
30
Annealing
59 ˚C
30 sec
Extension
72 ˚C
30 sec
3
Final extension
72 ˚C
5 min
1
Table 4: COMT rs 165599 PCR condition
Table 5
Size bp
Repeat (R) No.
control
case
OR
95% CI
P.
350
3R
41
23
1.00
--------
------
320
2R
5
2
0.713
0.128-3.972
0.698
320/350
2R/3R
2
23
20.5
4.428-94.907
0.001
380
4R
2
1
0.891
0.077-10.372
1.0
Table 5: Genotypes distribution of MAOA uVNTR polymorphism with allele frequency and their association in control and case groups
OR = Odds ratio 95 % CI = 95 % confidence interval P = probability value
Table 6
Val (G)
Met (A)
OR
95% CI
P.
control
33
17
1.00
-------
-----
cases
25
25
1.9411
0.939-4.71
0.069
Table 6: Allele frequency and their association in control and cases groups for COMT gene polymorphism rs4680
Table 7
C
%
T
%
OR
95% CI
P.
control
29
58%
21
42%
1.00
------
------
cases
25
50%
25
50%
0.75
0.34-1.656
0.476
Table 7: Allele frequency and their association in control and cases groups for COMT gene polymorphism rs 165599
C= normal, T= polymorphism
Table 8
W
p
O.R
95% CI
P.V.
control
23
27
1.00
----
-------
Cases
10
40
3.407
1.401-8.285
0.00569
Table 8: polymorphism at MAOA promoter VNTR (2R/3R), COMT rs4680, and COMT rs 165599 in two groups
W= Wild genotype (without any polymorphism), P= (2R/3R) uVNTR at MAOA gene promoter or at least one polymorphism in COMT rs4680 or rs165599 in participants
Table 9
MAOA 2R/3R+ COMT rs 4680 rs165599
control
Cases
OR
95% CI
P.V.
No polymorphism
21
10
1.00
---------
--------
Three polymorphism
1
10
21
2.352 -187.494
0.00081
Table 9: COMT rs165599, COMT rs4680, and MAOA 2R/3R are in the same participant compared to those who do not have any mutation or only one or two mutations
FIGURE 1
Figure 1: MAOA gene is located on the short (p) arm of the X chromosome at position 11.3
FIGURE 2
Figure 2: The COMT gene structure
FIGURE 3
Figure 3: The Electrophoresis pattern of PCR product for MAOA gene this amplification product was representing the VNTR polymorphism for gene in 2.5% agarose, 75 V, for 2 h (Lane L: DNA ladder (100bp) Lane 1, 2 & 3: band 320/350 bp, represent the heterozygote’s genotype 2/3 repeat. Lane 4: band 350/380 bp, represent the heterozygote’s genotype 3/4 repeat. Lane 5 & 6: band 350 bp, represent the homozygote’s genotype 3 repeat
FIGURE 4
Figure 4: tow repeat (R) sequences in MAOA gene promoter are colored by blue and red, from Gen Bank(Accession number M89636.1)[29]
FIGURE 5
Figure 5: The Electrophoresis pattern of PCR product for COMT gene rs4680 this amplification products were representing for the gene in 2.5% agarose, 75 V, for one h
Figure 7(b): Sequence of COMT rs 4680 showing G mutation to A
FIGURE 8
Figure 8: Sequences alignment results for cases and control for COMT rs4680 gene fragment. Revealed the substitution of a guanine to adenine G>A in samples 4, 6, 8, 9, 10.11, and12 for case group
FIGURE 9
Figure 9: Reverse sequence of COMT rs 165599 showing C > A
FIGURE 10
Figure 10: COMT rs 165599 sequence aliment using Jalview program with polymorphism (C>T) at control samples. Revealed the substitution of cytosine to thymine C>T in 4, 8, and 11samples
Tables at a glance
Figures at a glance