Tables at a glance

Table 1

Table 2

Table 3

Table 4

Table 5

Table 6

Table 7

Table 8

Table 9

Figures at a glance

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

Figure 8

Figure 9

Figure 10

Figure 11

Table 1

NO.	Reagent	Volume
1	DNA template	2 μl
2	Forward primer	1 μl
3	Reverse primer	1 μl
4	Bioneer master mix	5 μl
5	Nuclease-free water	16 μl
Total volume		25 μl

Table 1: Reagents of PCR amplification (25 ul) for MAOA gene

Table 2

Stage No.	steps	Temperature	Time	No. of cycles
1	Initial denaturation	95 ˚C	5 min	1
2	Denaturation	94 ˚C	30 sec	30
	Annealing	60 ˚C	30 sec
	Extension	72 ˚C	30 sec
3	Final extension	72 ˚C	5 min	1

Table 2: The program used in PCR amplification of MAOA gene

Table 3

Stage No.	steps	Temperature	Time	No. of cycles
1	Initial denaturation	95 ˚C	5 min	1
2	Denaturation	94 ˚C	30 sec	30
	Annealing	60 ˚C	30 sec
	Extension	72 ˚C	30 sec
3	Final extension	72 ˚C	5 min	1

Table 3: COMT rs 4680 PCR condition

Table 4

Stage No.	steps	Temperature	Time	No. of cycles
1	Initial denaturation	95 ˚C	5 min	1
2	Denaturation	94 ˚C	30 sec	30
	Annealing	59 ˚C	30 sec
	Extension	72 ˚C	30 sec
3	Final extension	72 ˚C	5 min	1

Table 4: COMT rs 165599 PCR condition

Table 5

Size bp	Repeat (R) No.	control	case	OR	95% CI	P.
350	3R	41	23	1.00	--------	------
320	2R	5	2	0.713	0.128-3.972	0.698
320/350	2R/3R	2	23	20.5	4.428-94.907	0.001
380	4R	2	1	0.891	0.077-10.372	1.0

Table 5: Genotypes distribution of MAOA uVNTR polymorphism with allele frequency and their association in control and case groups
OR = Odds ratio 95 % CI = 95 % confidence interval P = probability value

Table 6

	Val (G)	Met (A)	OR	95% CI	P.
control	33	17	1.00	-------	-----
cases	25	25	1.9411	0.939-4.71	0.069

Table 6: Allele frequency and their association in control and cases groups for COMT gene polymorphism rs4680

Table 7

	C	%	T	%	OR	95% CI	P.
control	2 9	58%	21	42%	1.00	------	------
cases	25	50%	25	50%	0.75	0.34-1.656	0.476

Table 7: Allele frequency and their association in control and cases groups for COMT gene polymorphism rs 165599
C= normal, T= polymorphism

Table 8

	W	p	O.R	95% CI	P.V.
control	23	27	1.00	----	-------
Cases	10	40	3.407	1.401-8.285	0.00569

Table 8: polymorphism at MAOA promoter VNTR (2R/3R), COMT rs4680, and COMT rs 165599 in two groups
W= Wild genotype (without any polymorphism), P= (2R/3R) uVNTR at MAOA gene promoter or at least one polymorphism in COMT rs4680 or rs165599 in participants

Table 9

MAOA 2R/3R+ COMT rs 4680 rs165599	control	Cases	OR	95% CI	P.V.
No polymorphism	21	10	1.00	---------	--------
Three polymorphism	1	10	21	2.352 -187.494	0.00081

Table 9: COMT rs165599, COMT rs4680, and MAOA 2R/3R are in the same participant compared to those who do not have any mutation or only one or two mutations

FIGURE 1

Figure 1: MAOA gene is located on the short (p) arm of the X chromosome at position 11.3

FIGURE 2

Figure 2: The COMT gene structure

FIGURE 3

Figure 3: The Electrophoresis pattern of PCR product for MAOA gene this amplification product was representing the VNTR polymorphism for gene in 2.5% agarose, 75 V, for 2 h (Lane L: DNA ladder (100bp) Lane 1, 2 & 3: band 320/350 bp, represent the heterozygote�s genotype 2/3 repeat. Lane 4: band 350/380 bp, represent the heterozygote�s genotype 3/4 repeat. Lane 5 & 6: band 350 bp, represent the homozygote�s genotype 3 repeat

FIGURE 4

Figure 4: tow repeat (R) sequences in MAOA gene promoter are colored by blue and red, from Gen Bank(Accession number M89636.1)[29]

FIGURE 5

Figure 5: The Electrophoresis pattern of PCR product for COMT gene rs4680 this amplification products were representing for the gene in 2.5% agarose, 75 V, for one h

FIGURE 6

Figure 6: electropherogram showing COMT rs 4680 polymorphism sequence with mutation (G>A)

FIGURE 7

Figure 7(a): Reference sequence of COMT rs 4680

Figure 7(b): Sequence of COMT rs 4680 showing G mutation to A

FIGURE 8

Figure 8: Sequences alignment results for cases and control for COMT rs4680 gene fragment. Revealed the substitution of a guanine to adenine G>A in samples 4, 6, 8, 9, 10.11, and12 for case group

FIGURE 9

Figure 9: Reverse sequence of COMT rs 165599 showing C > A

FIGURE 10

Figure 10: COMT rs 165599 sequence aliment using Jalview program with polymorphism (C>T) at control samples. Revealed the substitution of cytosine to thymine C>T in 4, 8, and 11samples

FIGURE 11

Figure 11: electropherogram showing the genotype polymorphism COMT rs 165599 sequence