
Figure 1: a) Whole Genome Sequencing: The starting is from creating a microorganism colony. DNA is extracted, fragmented, and stored in a library for Sanger sequencing or other NGS procedures. The library is sequenced and examined using bioinformatics pipelines. (b) Targeted Sequencing: This procedure starts with a clinical sample and involves selection or enrichment before library preparation for bacteria and fungus. If the pathogen of interest is a virus, selection or enrichment takes place following library preparation. After library preparation, materials are sequenced and analyzed using a bioinformatics process [22].
Figures at a glance