Neoplasm	Mean NS5	Median NS	Pleomorphism6	Cellularity
ISK1	117	110	57	398
VV2	216	201	92	127
HAK3	223	210	111	83
SCC4	231	218	126	66

1: Irritated seborrheic keratosis 2: Verruca vulgaris 3: Hypertrophic actinic keratosis 4: Squamous cell carcinoma 5: Nuclear Size 6: Pleomorphism measured via the standard deviation of the nuclear size

Table 1: Image Analysis Characteristics of the Known Neoplasms

Neoplasm	NS5 Range6	Pleomorphism Range7	Cellularity Range8
ISK1	61-173	49-65	200-596
VV2	124-307	74-110	52-202
HAK3	112-334	89-133	50-116
SCC4	105-357	95-157	32-100

1: Irritated seborrheic keratosis 2: Verruca vulgaris 3: Hypertrophic actinic keratosis 4: Squamous cell carcinoma 5: Nuclear Size 6: Created by taking the NS +/- 1 standard deviation 7: Created by taking the NS standard deviation +/- 1 standard deviation 8: Created by taking the Cellularity +/- 1 standard deviation

Table 2: Diagnostic Ranges

Case	Mean NS1	Possibilities	IA Diagnosis	Clinical Correlation
Case 1	115	ISK2	Benign	Benign
Case 2	297	VV3, HAK4, SCC5	Indeterminate *	Benign
Case 3	536	SCC	Malignant	Malignant
Case 4	209	VV, HAK, SCC	Indeterminate	Benign
Case5	300	VV, HAK, SCC	Indeterminate	Benign
Case 6	267	VV, HAK, SCC	Indeterminate	Malignant
Case 7	342	SCC	Malignant	Malignant
Case 8	355	SCC	Malignant	Benign
Case 9	197	VV, HAK, SCC	Indeterminate	Benign
Case 10	412	SCC^	Malignant	Malignant

1: Nuclear Size 2: Irritated seborrheic keratosis 3: Verruca vulgaris 4: Hypertrophic actinic keratosis 5: Squamous cell carcinoma *: When cases could not be reliably classified as Benign or Pre-malignant/Malignant, they were classified as Indeterminate. ^: In instances where the NS exceeded the highest value of the NS diagnostic ranges, the IA diagnosis rendered was that of SCC. Blue: Designates an IA diagnosis that correctly correlates with the clinical course. Red: Designates an IA diagnosis that incorrectly correlates with the clinical course.

Table 3a: Diagnosing Transected Neoplasms using the Nuclear Size Diagnostic Range

Case	Pleomorpism	Possibilities	Plemorphism Diagnosis	Clinical Correlation
Case 1	42	ISK1*	Benign	Benign
Case 2	83	VV2	Benign	Benign
Case 3	191	SCC3	Malignant	Malignant
Case 4	50	ISK4	Benign	Benign
Case5	94	VV, HAK	Indeterminate	Benign
Case 6	108	VV, HAK, SCC	Indeterminate	Malignant
Case 7	125	HAK, SCC	Malignant	Malignant
Case 8	65	ISK	Benign	Benign
Case 9	82	VV	Benign	Benign
Case 10	144	SCC	Malignant	Malignant

1: Irritated seborrheic keratosis 2: Verruca vulgaris 3: Hypertrophic actinic keratosis 4: Squamous cell carcinoma *: In instances where the pleomorphism fell below the lowest value of the Pleomorphism diagnostic ranges, the IA diagnosis rendered was that of ISK.

Table 3b: Diagnostic Ranges

Case	Cellularity	Possibilities	Cellularity Diagnosis	Clinical
Case 1	215	ISK1	Benign	Benign
Case 2	42	SCC	Malignant	Benign
Case 3	36	SCC	Malignant	Malignant
Case 4	93	VV2, HAK3, SCC4	Indeterminate	Benign
Case5	86	VV, HAK, SCC	Indeterminate	Benign
Case 6	108	VV	Benign	Malignant
Case 7	71	VV, HAK, SCC	Indeterminate	Malignant
Case 8	69	VV, HAK, SCC	Indeterminate	Benign
Case 9	216	ISK	Benign	Benign
Case 10	63	VV, HAK, SCC	Indeterminate	Malignant

1: Irritated seborrheic keratosis 2: Verruca vulgaris 3: Hypertrophic actinic keratosis 4: Squamous cell carcinoma

Table 3c: Diagnosing Transected Neoplasms using the Cellularity Diagnostic Range

Figure 1: Example of Image Anaysis performed on a Known Neoplasm 1a: H&E image of a known hypertrophic actinic keratosis neoplasm (original magnification x 20). 1b: A representative area of stratum spinosum is selected comprising 210,000 pixels. 1c: Nuclei are selected out. 1d: Image analysis carried out using Image J software.