
Figure 1: 2D and 3D cellular culture systems. In the 2D system, cells adhere to the plastic, and are visualized usually as monolayers attached to the cell culture plate surface (1A). In the 3D system, cells are structured as a sphere, which commonly grows in suspension in the medium. There are three different regions in these spheroids: necrotic (1), quiescent (2) and proliferating (3) zones (1B). Ultra-Low Attachment plates or Matrigel-coated plates are usually used for 3D cell culture
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