Figure 2 Production of G-CSF, IL-2, IL-6, and IFN-γ in culture supernatants from ABQ 48-treated lymphocyte cultures. Murine splenic lymphocytes were isolated and incubated with twofold serial dilutions of ABQ-48 (5-0.3 ug/mL) for 5 days. Culture supernatants were collected and the cytokine concentrations were determined by protein microarray analysis. Supernatants from Concanavalin A-treated lymphocytes, and media alone were used as positive and negative controls, respectively. Results were expressed as mean concentrations ± SEM of three independent experiments.

Figure 3 Production of G-CSF, IL-2, IL-6, and IFN-γ in culture supernatants from NBQ 48-treated lymphocyte cultures. Murine splenic lymphocytes were isolated and incubated with twofold serial dilutions of NBQ-48 (5-0.3 μg/mL) for 5 days. Culture supernatants were collected and the cytokine concentrations were determined by protein microarray analysis. Supernatants from Concanavalin A-treated lymphocytes, and media alone were used as positive and negative controls, respectively. Results were expressed as mean concentrations ± SEM of three independent experiments.

ABQ-48, μg/mL	Cytokine	Cytokine concentration, pg/mL±SEM
0.6	G-CSF	32.3±22.8
0.3	IL-2	18.5±7.2
1.2	IL-6	61.9±20.4
5.0	IFN-	21.7±12.8

Table1 Maximal concentrations of cytokines produced in culture supernatants from ABQ-48-treated lymphocyte cultures. Murine splenic lymphocytes were isolated and incubated with twofold serial dilutions of ABQ-48 (5-0.3 µg/mL) for 5 days. Culture supernatants were collected and the cytokine concentrations were determined by protein microarray analysis. Supernatants from Concanavalin A-treated lymphocytes, and media alone were used as positive and negative controls, respectively. Results were expressed as mean concentrations  SEM of three independent experiments.

NBQ-48, μg/mL	Cytokine	Cytokine concentration, pg/mL±SEM
0.6	G-CSF	67.6±57.5
2.5	IL-2	37.2±15.1
5.0	IL-6	65.2±40.2
0.6	IFN-	3.8±3.8

Table2 Maximal concentrations of cytokines produced in culture supernatants from NBQ-48-treated lymphocyte cultures. Murine splenic lymphocytes were isolated and incubated with twofold serial dilutions of NBQ-48 (5-0.3 μg/mL) for 5 days. Culture supernatants were collected and the cytokine concentrations were determined by protein microarray analysis. Supernatants from Concanavalin A-treated lymphocytes, and media alone were used as positive and negative controls, respectively. Results were expressed as mean concentrations ± SEM of three independent experiments.