Figure 1 Expression of MyoD1 in rhabdomyosarcoma, neuroblastoma, and other tumor cell lines

Figure 2 PAX3/FKHR chimera gene in alveolar rhabdomyosarcoma cell lines

Figure 3 Cytoskeletal protein of SCMC-RM2 rhabdomyosarcoma cell line

Figure 4 MYCN-amplification (a) and expression (b) in SCMC-RM2 rhabdomyosarcoma cell line

Figure 5 Establishment of the MP-ASKIN-SA cell line from the left pulmonary thoracopulmonary tumor from a 13 yr-old boy

Figure 6 EWS/FLl1 chimera gene (a) and c-myc expression (b) in the MP-ASKIN-SA cell line

Figure 7 Inverted microscopic (a) and electron microscopic (b) analysis of the KP-MRT-NS cell line

Figure 8 Indirect immunofluorescence with (a) neurofilament, (b) alpha-smooth-muscle actin, and (c) SM1 isoforms of the smooth-muscle-myosin heavy chain in the KP-MRT-NS cell line

Figure 9 Western blot analysis of cytoplasmic proteins of KP-MRT-NS cell line with neurofilament, alpha-smooth-muscle, basic calponin and SM1 isoforms.

Figure 10 Chest XP and abdominal computed tomographic imaging of an established KP-MRT-RY cell line

Figure 11Abdominal computed tomographic image showing massive pleural fluid and multiple hepatic tumor lesions in an established MP-MRT-AN cell line

Figure 12 Chest computed tomographic image showing an enhanced thoracic mass on admission in an established KP-MRT-YM cell line

Figure 13 INI1 gene analysis in four MRT cell lines

Figure 14 Effect of gefitinib on two MRT cell lines

Figure 15 Inhibition of tumor growth by gefitinib in MRT cell lines

Figure 16 Trastuzumab enhances cytotoxicity against MRT cell lines using allogenic human peripheral blood mononuclear cells

Figure 17 Trastuzumab enhances cytotoxicity against KP-MRT-YM cells

Figure 18 Enhanced computed tomography scan of left and right Achilles tendons of a patient with clear cell sarcoma of soft parts

Figure 19 Electron microphotograph of MP-CCS-SY cell line

Figure 20 Immunofluorescence and western blot with HMB45 and Melan-A antibodies in MP-CCS-SY cell line

Figure 21 EWS/ATF1 chimeric gene (a) and c-myc mRNA in MP-CCS-SY cell line and SY tumor cells

No	Cell line	Age/gender	Primary tumor	Stage	Culture Sample	Outcome	References
Rhadomyosarcoma
1	SCMC-RM2	10y/F	Rt-abdominal wall	IV	Bone marrow	Dead	11
2	KP-RMS-DH	14y/M	Lt-mandibular cavity	IV	Bone marrow	Dead	Unpublished
3	KP-RMS-KH	14/F	Perinea	IV	Primary tumor	Dead	Unpublished
Ewing sarcoma family tumor
1	KP-EW-YI	16y/M	Rt-pelvis	II	Primary tumor	Dead	17
2	KP-EW-MS	16y/F	Rt-breech	IV	Bone marrow	Dead	17
3	MP-ASKIN-SA	13y/M	Lt-chest	II	Primary tumor	Dead	18
4	KP-PNET-TO	15y/M	Lt-kidney	IV	L-iliac	Dead	Unpublished
5	KP-EW-AK	15y/M	Lt-scapula	IV	Primary tumor	Dead	Unpublished
Malignant rhabdoid tumor
1	KP-MRT-NS	2m/F	Lt-kidney	II	Ascites	Dead	19, 20, 24
2	KP-MRT-RY	1m/M	Lt-kidney	I	Primary tumor	Dead	19, 20, 22, 23
3	MP-MRT-AN	3m/F	liver	IV	Peripheral blood	Dead	20, 21, 24
4	KP-MRT-YM	5m/M	Rt-chest wall	I	Primary tumor	Alive	20, 22, 23
Clear cell sarcoma of soft parts
1	MP-CCS-SY	13y/F	Lt-Achillis	II	Lt-popliteall fossa	Dead	26, 28

Table1 Our established soft-tissue-sarcoma cell lines from children