Figure 1: Calibration curves for Doxorubicin (A) and Vincristine (B)
Time (min) |
Eluent A |
Eluent B |
2 |
97 |
3 |
2.50 |
97 |
3 |
10 |
76 |
24 |
13.0 |
76 |
24 |
13.50 |
66 |
34 |
15.50 |
44 |
56 |
16.00 |
97 |
3 |
21.00 |
97 |
3 |
Table 1: Eluent A: Water / 0.1% formic acid; Eluent B: Acetonitrile / 0.1% formic acid
Doxorubicin Chlorohydrate |
330 g/ml |
|
Vincristine sulfate |
11.5 g/ml |
|
Diluent |
Normal Saline |
|
Storage condition |
25° C |
|
Determinations |
0 h |
48 h |
Table 2: Quantitative and qualitative conditions of analyses for Doxorubicin/Vincristine mixture
Concentration |
4.86 mg/mL |
55.36 mg/mL |
57.86 mg/mL |
||||
Diluent |
Normal Saline |
Normal Saline |
Normal Saline |
||||
Storage condition |
25° C |
25° C |
25° C |
32° C |
|||
Determinations |
0 h |
21 d |
0 h |
21 d |
0 h |
28 d |
35 d |
Table 3: Quantitative and qualitative conditions of analyses for 5-FU.
Temperature |
Time |
Average concentration |
Average Concentration |
25° C |
0 h |
330±0.07 |
- |
25° C |
48 h |
|
|
Table 4: LC-MS stability of Doxorubicin and Vincristine solution at Time = 48 h
Figure 1: Calibration curves for Doxorubicin (A) and Vincristine (B)
Figure 2A-B: HPLC and LC/MS chromatograms for Doxorubicin (A) and Vincristine (B)
b) 5-Fluorouracil (5-FU)
Figure 3: Calibration curve for 5-FU
Figure 4A-D: Chromatograms of Doxorubicin and Vincristine mixture at T =0 and T = 48 h at 25° C
Figure 5A-D: Representative chromatograms of two different 5-FU concentrations at T=0 and T= 21 days
Figure 6A: Representative chromatograms of 5-FU 57.86 mg/mL solution at 28 days and 35 days
Tables at a glance
Figures at a glance