
Figure 1: The PDGF-induced proliferation of hVSMCs is DNA-PK dependent. (A) Proliferation assay of hVSMCs treated with the DNA-PK inhibitor NU7026 (1 µmol/l) and PDGF-BB (10 ng/ml); BrdU incorporation was quantified. The fold induction is the ratio of stimulated to unstimulated cells. ***p < 0.001 (B) Immunoblot analysis of cyclin D1expression in hVSMCs after PDGF treatment and DNA-PK inhibition by the DNA-PK inhibitor NU7026; vinculin was used as a loading control. (C) mRNA expression of cyclin D1 in hVSMCs incubated for 6, 18, 24, and 48 hours with PDGF-BB (10 ng/ml) in the absence or presence of the DNA-PK inhibitor NU7026 (1 µmol/l). The fold induction is the ratio of stimulated to unstimulated cells, and error bars represent the S.D.s of three experiments (four measurements) *p < 0.05.