Figure 1

		Frequency	Percentage
Diagnosis	Benign	17	34
	Premalignant	21	42
	Malignant	12	24
	Total	50	100

Table I: Percentage and frequency of the histological diagnosis

Nº	Case	Diagnosis	Ki67	P16	P21	P53	e-cadherin	Gender
1	7353	Benign	YES	YES	YES	NO	+++	F
2	25654	Benign	NO	NO	NO	NO	+++	F
3	8100	Benign	YES	NO	YES	NO	+++	M
4	101	Benign	YES	NO	YES	NO	+++	M
5	289	Benign	NO	NO	NO	NO	+++	F
6	324	Benign	YES	NO	NO	NO	+++	F
7	351	Benign	NO	NO	NO	NO	+++	F
8	500	Benign	NO	NO	NO	NO	+++	F
9	564	Benign	YES	YES	NO	NO	+++	F
10	808	Benign	YES	NO	NO	NO	+++	M
11	809	Benign	YES	NO	NO	NO	+++	M
12	9732	Benign	YES	NO	NO	NO	+++	F
13	10197	Premalign	YES	NO	NO	NO	+++	F
14	10219	Premalign	YES	NO	YES	NO	+++	F
15	10278	Premalign	YES	NO	NO	NO	+++	F
16	28875	Premalign	YES	YES	YES	YES	+++	F
17	3296	Premalign	YES	NO	NO	NO	+++	F
18	1619	Premalign	YES	YES	NO	NO	+++	M
19	6313	Premalign	YES	YES	YES	YES	+++	F
20	6513	Premalign	YES	YES	YES	NO	+++	M
21	9196	Premalign	NO	YES	NO	NO	+++	M
22	174	Premalign	NO	NO	NO	NO	+++	F
23	2635	Premalign	NO	NO	NO	NO	+++	M
24	2857	Premalign	YES	NO	NO	NO	+++	M
25	6426	Premalign	YES	NO	NO	NO	+++	F
26	9740	Premalign	YES	NO	NO	NO	+++	M
27	23143	Premalign	YES	YES	NO	NO	+++	M
28	23144	Premalign	YES	NO	YES	NO	+++	F
29	1623	Premalign	YES	NO	NO	NO	+++	F
30	4729	Premalign	NO	NO	NO	NO	+++	F
31	9733	Premalign	YES	NO	YES	NO	+++	M
32	23096	Premalign	YES	YES	YES	NO	+++	M
33	23865	Premalign	NO	NO	NO	NO	+++	M
34	4839	Malignant	NO	NO	NO	NO	+++	F
35	8973	Malignant	NO	YES	NO	YES	+++	F
36	5147	Malignant	YES	YES	YES	YES	+++	F
37	7542	Malignant	YES	NO	NO	YES	+++	M
38	8821	Malignant	YES	NO	NO	YES	+++	F
39	1445	Malignant	YES	NO	NO	NO	+++	M
40	2403	Malignant	YES	YES	YES	NO	+++	F
41	4466	Malignant	YES	YES	NO	NO	+++	F
42	7946	Malignant	YES	NO	YES	YES	+++	M
43	23145	Malignant	YES	NO	YES	NO	+++	F
44	2397	Malignant	YES	YES	YES	NO	+++	F
45	5374	Malignant	YES	YES	YES	NO	+++	F
46	6099	Malignant	YES	NO	YES	YES	+++	F
47	6688	Malignant	YES	YES	YES	YES	+++	F
48	6948	Malignant	YES	NO	NO	NO	+++	F
49	8335	Malignant	YES	NO	YES	YES	+++	M
50	8870	Malignant	YES	NO	YES	YES	+++	M

Table 2: Immunohistochemical and histological results, referring each case. F=female; M=male.

Contingency Table
			Diagnosis		Total
			Benign	Malignant	Total
p16	No overexpression	Count	24	10	34
	No overexpression	p16 %	70,6%	29,4%	100%
	Overexpression	Count	9	7	16
	Overexpression	p16 %	56,3%	43,8%	100%
Total		Count	33	17	50
Total		p16 %	66%	34%	100%

Table 3: contingency table for protein p16.