Figure 1:Strong anion exchange chromatography (Q-Sepharose Fast Flow) of B. aegyptiaca extract treated with 10% (w/v) charcoal. The Q-Sepharose column was pre-equilibrated with 50 mM Tris-HCl buffer (pH 8.1), containing 1 mM EDTA and 3 mM β-mercaptoethanol. Experiment was carried out with this buffer. Elution was performed using the same buffer containing 1 M NaCl at a flow rate of 0.5 mL/min under landing gradient of 0, 0.15, 0.4 and 1 M. (P corresponding to peak)
Tables at a glance
Figures at a glance