Tables at a glance

Table 1

Table 2

Table 3

Table 4

Table 1

Target gene	Primer Sequenza 5’ – 3’	Amplicon size (bp)	Concentration (µM)	Reference
A16S F	GGG AGT GCC TTC GGG AAT CAG A	356 bp	0.24	Wang et al, 2003
A16S R	TCA CCG CAA CAT TCT GAT TTG		0.24
A-hyd F	AGT CTG CCG CCA GTG GC	144 bp	0.48	S.Persson et al,2015
A-hyd R	CRC CCA TCG CCT GTT CG		0.48	S.Persson et al,2015
astF	ATG CAC GCA CGT ACC GCC AT	260 bp	0.12	Kingombe et al,2010
astR	ATC CGG TCG TCG CTC TTG GT		0.12	Kingombe et al,2010
lipF	ATC TTC TCC GAC TGG TTC GG	382 bp	0.8	K.SenandM.Rodgers, 2004
lipR	CCG TGC CAG GAC TGG GTC TT		0.8	K.SenandM.Rodgers, 2004
elaF	ACA CGG TCA AGG AGA TCA AC	513 bp	0.2	K.SenandM.Rodgers, 2004
elaR	CGC TGG TGT TGG CCA GCA GG		0.2	K.SenandM.Rodgers, 2004
	GAG AAG GTG ACC ACC AAG AACA	232 bp	0.8	Kingombe et al,2010
actF	GAG AAG GTG ACC ACC AAG AACA			Kingombe et al,2010
actR	AAC TGA CAT CGG CCT TGA ACT C		0.8
altF	GCA CGG CGT GAC TTC GGT GA	576 bp	0.8	Kingombe et al,2010
altR	ACC GCG GTC TTG CAG TTG GG		0.8	Kingombe et al,2010
aerF	AAC CGA ACT CTC CAT	301 bp	1.2	Pabbs et al, 2009
aerR	CGC CTT GTC CTT GTA		1.2
	GGC CGG TGG CCC GAA GAT ACGGG	597 bp	0.12	Wong et al, 1998
hlyAF	GGC CGG TGG CCC GAA GAT ACGGG
hlyAR	GGC GGC GCC GGA CGA GAC GGG		0.12

Table 1: PCR Primers for Aeromonas genus-specific and virulence associated

Table 2

Multiplex 1		Multiplex 2		Multiplex 3
dNTPs	0,5 µl	dNTPs	0,5 µl	dNTPs	0,5 µl
A16S-F	0,5 µl	alt-F	2 µl	hlyA-F	0,3 µl
A16S-R	0,5 µl	alt-R	2 µl	hlyA- R	0,3 µl
A-hyd -F	0,5 µl	lip-F	2 µl	ela-F	0,5 µl
A-hyd -R	0,5 µl	lip-R	2 µl	ela-R	0,5 µl
		ast-F	0,3 µl	aer-F	3 µl
		Ast-R	0,3 µl	aer-R	3 µl
				act-F	2 µl
				act-R	2 µl
H2O PCR	14,2 µl		9,2 µl		6,2 µl
DNA template	2 µl		2 µl		2 µl
Taq Polymerase	0,2 µl		0,2 µl		0,2 µl
MgCl2	2 µl		2 µl		2 µl
Buffer	2,5 µl		2,5 µl		2,5 µl
Volume totale	25 µl		25 µl		25 µl

Table 2: Mix Reaction Multiplex PCR 1,2,3

Table 3

Step	Temperature	Time (Min)	Cycle
Initial denaturisation	95°C	0.208333333	1
Denaturisation	95°C	0.020833333	30
Coupling	60°C	0.020833333
Extension	72°C	0.041666667
Final Extension	72°C	0.291666667	1

Table 3: Multiplex 1,2,3 PCR: thermic profiles

Table 4

		Alt	Lip	Ast	Hly	Ela	Aer	Act
1C	A. h	+	+	-	+	+	-	-
2C	A. spp	-	-	-	-	-	-	+
3C	A. spp	-	+	-	+	-	-	+
4C	A. h	+	+	+	+	+	-	-
5C	A. h	+	+	-	-	+	-	+
6C	A. h	+	+	-	+	+	-	-
7C	A. spp	-	+	-	-	+	-	-
8C	A. h	+	+	-	+	+	-	-
9C	A.spp	-	+	-	+	-	-	+
10C2	A. spp	-	-	-	-	-	-	+
10C1	A. h	+	+	+	-	+	-	-
13C	A. spp	-	+	-	-	+	-	-
11C	A. h	+	+	-	+	+	-	-
12C	A. h	+	+	+	+	+	-	-
14C	A. spp	-	+	-	-	+	-	-
17C	A. spp	-	+	-	+	-	-	+

Table 4: Detection enterotoxin gene in isolates of Aeromonas hidrophila and spp in the meat samples