Tables at a glance

Table 1

Table 2

Table 3

Table 4

Figures at a glance

Figure 1

Figure 2

Table 1

Compound	Molecular weight (mg/ml)
Anthocyanins	253.3
Gallic acid	193.0
Tannins	31.6
Ellagic acid	94.7

Table 1: Quantification of various compounds in Punica granatum extract

Table 2

Late apoptotic cells (%)	Early apoptotic cells (%)	necrotic cells (%)	Live cells (%)	Treatment
8.39	4.83	1.09	87.5	Control
5.48	45	4.12	49.1	Extract (8 µg/ml)
6.10	19.3	1.28	73.3	AFB1 (0.05 µg/ml)
9.66	17.3	1.92	71.1	Ochra (3.5 µg/ml)
6.55	23.2	4.97	65.3	AFB1 (0.05 µg/ml) and extract (8 µg/ml)
11.8	38.1	6.02	44.1	Ochra (3.5 µg/ml) and extract (8 µg/ml)

Table 2: Percentages of live, necrotic, early, and late apoptotic cancer cells treated with Punica granatum extract, AFB1, Ochra, extract and AFB1, and extract and Ochra at designated doses using flow cytometry

Table 3

TLR4	TLR2	Treatment
0.85±0.21	1.35±0.98	Control
0.82±0.12	1.28±0.33	Extract (8 µg/ml)
1.93±0.43	2.23±1.09	AFB1 (0.05 µg/ml)
1.41±0.78	2.23±1.09	Ochra (3.5 µg/ml)
1.84±0.91	1.82±0.62	AFB1 (0.05 µg/ml) and extract (8 µg/ml)
1.40±0.32	1.88±0.63	Ochra (3.5 µg/ml) and extract (8 µg/ml)

Table 3: Expressions of TLR2 and TLR4 genes in A549 human lung adenocarcinoma cell lines treated with P. granatum extract, extract and Ochra, extract and AFB1, Ochra, and AFB1 at designated doses using qPCR (mean± SD). SD: standard deviation

Table 4

TSLP (pg/ml)	IL-33 (pg/ml)	IL-25 (pg/ml)	Treatment
0.13±0.10	0.09±0.09	0.017±0.09	Control
0.19±0.16	0.12±0.43	0.041±0.022	Extract (8 µg/ml)
1.80±1.05	1.20±0.81	0.63±0.90	AFB1 (0.05 µg/ml)
0.19±0.15	1.20±0.051	0.21±0.11	Ochra (3.5 µg/ml)
0.57±0.92	0.70±0.21	0.19±0.75	AFB1 (0.05 µg/ml) and extract (8 µg/ml)
0.12±0.23	0.70±0.02	0.05±0.017	Ochra (3.5 µg/ml) and extract (8 µg/ml)

Table 4: Mean±standard deviation (SD) values of Th2-promoting cytokines in A549 human lung adenocarcinoma cell line cultures treated with P. granatum extract, extract and Ochra, extract and AFB1, Ochra, and AFB1 at designated doses for 24 hours using ELISA

FIGURE 1

Figure 1: Chromatogram analysis of the ethanolic extract from P. granatum seed using HPLC/MS

FIGURE 2

Figure 2: Induction of necrosis and apoptosis in A549 human lung adenocarcinoma cell line using various treatments. (A) A549 cells were exposed to P. granatum extract, extract and Ochra, extract and AFB1, Ochra, and AFB1 at designated doses for 24 hours. (B) The necrosis, early, and late apoptosis rates were statistically treated. Data were expressed as mean±SD. (P < 0.05, compared to control). Ochra: Ochratoxin A; AFB1: Aflatoxin B1; ctrl: Control