Figure 1 TEM images of CNP vehicles (a,b) and BSA-loaded CNP (c-f) in water (a,c,e) or 1.7 mg/mL lysozyme (b,d,f). Scale, 200 nm.
Figure 2 Release of bovine serum albumin (BSA) from chitosan nanoparticles suspended in PBS.
Figure 3Size distribution of chitosan nanoparticles after exposure to lysozyme.
Figure 4SDS-PAGE data of CNPs in water (lane 1), BSA in water (lane 2), BSA-CNP in water (lanes 3,4), lysozyme (lane 5), CNPs in lysozyme (lane 6), BSA in lysozyme (lane 7), and BSA-CNP in lysozyme (lanes 8,9).
Figure 5 Viability of rat osteosarcoma (ROS) (a) and MC3T3-E1 (b) cells exposed to chitosan nanoparticles.
Figure 6 Confocal micrographs of ROS (a-c) and MC3T3-E1 (d-f) cells exposed to Cy5.5-CNPs (red channel). The nuclei of the cells are visualized in the blue channel. Scale, 20 µm.
Table 1Physicochemical parameters of hydrophobically modified glycol chitosan nanoparticles. Hydrodynamic diameter and zetal potential were measured in DI water. Loading efficiency of BSA encapsulation was measured in PBS.
Chitosan:BSA massRatio |
Average |
Zeta |
Loading Efficiency (%) |
10:0 (vehicle) |
288.6 ± 21.8 |
13.2 ± 0.2 |
|
10:1 |
478.8 ± 9.6 |
12.7 ± 0.7 |
86.95 ± 2.90 |
10:2 |
374.8 ± 6.0 |
12.8 ± 0.4 |
89.91 ± 3.67 |