Figures at a glance

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FIGURE 1

Figure 1: Inhibitory effects of ART on U251 cells. ART could suppress the growth of U251 glioma cells. (A) Morphological changes were observed in ART-treated glioma cells; (B) ART resulted in a comparable dose- and time- dependent decrease in cell viability

FIGURE 2

Figure 2: ART inhibits U251 cell proliferation. Cell proliferation was tested by MTT assay. The proliferation rate of U251 cells was inhibited treated with ART at 400ug/ml compared with control group (n = 6, * P < 0.01 compare to control group)

FIGURE 3

Figure 3: ART inhibits the invasion of U251 glioma cells in vitro. (A) The number of invasive cells was determined by the trans-well invasion assays and enumerated under the inverted microscope (100 ×); (B) The average number of invasion cells was calculated from 5 fields under the inverted microscope. (n = 5, *** P < 0.001)

FIGURE 4

Figure 4: ART induces U251 cell apoptosis. (A)The morphology of apoptotic cells relative to the normal cell morphology at a quantitative level (15000×). Apoptotic cells were analyzed in ART-treated cells after 48 hours drug treatment. These images were recorded by TEM. ? apoptotic body, ? high density chromatin, ? cell membrane is incomplete, ? cell vacuolation,? organelles cracking; (B) FCM method was used to analyze the apoptosis rate of cells in each group at a qualitative level; (C) statistical analysis showed there were significantly differences (n=3, P<0.001)

FIGURE 5

Figure 5: ART downregulates the expression of API5, c-MET, Bcl-2 and VEGF in U251 glioma cells. The expression levels of API5, c-MET, VEGF and Bcl-2 in ART-treated U251 cells were measured by western blot at 48 hours post drug treatment. The GAPDH was also measured as a reference (n = 3, * P<0.05, and ** P<0.01)