Figure 1:Outline of the study The genetic test of SLC25A13 examined the presence or absence of six kinds of mutations in the SLC25A13 gene: c.851_854delGTAT, c.1177+1G>A, c.1638_1660dup, c.674C>A, c.1230+1G>A, g.IVS16ins3kb.

Figure 2:: Questionnaire for specific food preferences of the 2013 survey versionWe defined the specific food preferences of these five food items and birth weight according to the questionnaire.

Figure 3:Questionnaire for specific food preferences of the 2014 and 2015 survey version. The numbers beside the boxes or parentheses are the scores for each answer. The scores were not shown in the real questionnaire. The total score was defined as the sum of the scores of the boxes and those of the parentheses for all 11 food items.

 

Total

2012 survey

2013 survey

2014 survey

2015 survey

N

16,468 (100.0)

1,345 (100.0)

3,969 (100.0)

6,952 (100.0)

4,202 (100.0)

Grade 2nd

4,451 (27.0)

317 (23.6)

1,098 (27.7)

1,867 (26.9)

1,169 (27.8)

4th

4,334 (26.3)

358 (26.6)

1,123 (28.3)

1,815 (26.1)

1,038 (24.7)

6th

4,336 (26.3)

375 (27.9)

1,014 (25.6)

1,784 (25.7)

1,163 (27.7)

8th

3,347 (20.3)

295 (21.9)

734 (18.5)

1,486 (21.4)

832 (19.8)

Boys

8,136 (49.4)

642 (47.7)

1,948 (49.1)

3,422 (49.2)

2,124 (50.6)

History of hospitalization 4,845

(29.4)

352 (26.2)

1,432 (36.1)

1,965 (28.3)

1,096 (26.1)

Birth weight, gram (N=14,849)

3,026 ± 450

No data

3,027 ± 432

3,023 ± 457

3,030 ± 457

Table1 Characteristics of the survey participants

Data are expressed as number (%) in categorical variable and mean ± standard deviation in continuous variable. History of hospitalization was defined hospitalization from any reason. Birth weight was not considered by gestational age.

No.

Sex

Age, y

Birth weight, gram*1

BMI,kg/m2

Easily fatigued

Neonatal jaundice*2

Poor hepatobiliary function*3

Survey year

Total score of questionnaire about food preferences*4

Gene mutation*5

1

Girl

15

3,100

18.8

Yes

No

No

2012

NA

Hetero [I]

2

Girl

13

2,600

16.8

No

No

No

2013

NA

No

3

Girl

10

2,900

18.1

No

No

No

2013

NA

No

4

Boy

14

2,200

14.5

No

No

No

2013

NA

No

5

Boy

8

2,900

14.5

No

Yes

No

2013

NA

No

6

Girl

9

2,500

15.3

No

Yes

No

2013

NA

No

7

Boy

10

2,200

15.9

No

Yes

Yes

2013

NA

No

8

Girl

15

3,300

24.7

No

No

No

2014

31

No

9

Girl

9

3,600

26.9

No

No

No

2014

42

No

10

Boy

15

3,100

28.2

Yes

Yes

No

2014

33

Hetero [II]

11

Boy

13

3,500

18.9

No

No

No

2014

28

No

12

Girl

8

1,700

13.4

No

Yes

No

2015

30

No

13

Boy

8

2,600

16.5

No

No

No

2015

30

No

Table2 PCR results and characteristics of the genetic test participants

*1 Birth weight was rounded to the nearest 100 and was not considered by gestational age. *2 Prolonged or sever symptom of neonatal jaundice or phototherapy for neonatal jaundice. *3 Poor hepatobiliary function in the neonatal period. *4 Total score was defined as sum of the point of the boxes and those of the parentheses for all the 11 food items in Fig. 3. *5 Mutation in the SLC25A13 gene. Hetero [I] and hetero [II] indicates heterozygous mutation of c.851_854delGTAT, and that of c.1177 + 1G>A, respectively.