Tables at a glance

Table 1

Table 2

Table 3

Table 4

Table 5

Table 6

Figures at a glance

Figure 1

Figure 2

Figure 3

Figure 4

Table 1

Phytochemical	Extract
Flavonoïdes	++
Tannins	++
Stérolsettriterpènes	-
Quinones	-
Saponines	+
Alcaloïdes	-

Table 1: Result of qualitative phytochemical composition of C. ladaniferus (L) extract

Bioavailability key; – = not detected, + = present in low concentration, ++ = present in moderately high concentration

Table 2

	Control	200mg/kg	400mg/kg	600mg/kg	800mg/kg	3200mg/kg
Respiration	N	N	N	N	N	Change
Food intake	N	N	N	N	N	Change
Immobility	N	N	N	N	N	Lethargy
Rigidity of the hind limbs	NP	NP	NP	NP	P	P
Body weight	N	N	N	N	N	Change
Change in skin	NC	NC	NC	NC	NC	N.O
Eyes	N	N	N	N	N	N.O

Table 2: Evaluation of the clinical signs of the aqueous leaf extract of C. ladaniferus intraperitoneally administered to mice

N= normal, N.P= not present, P= present, N.C= not change, NO= not observed
These signs disappeared after 48 hours. And at the end of the test, all the surviving mice return to their normal state.

Table 3

Doses(mg/kg)

wek1

wek2

wek3

wek4

wek5

wek6

wek7

wek8

wek9

wek10

wek11

wek12

0

170.1±27.2

178,22±27.2

181.7±26.6

184.9±27.2

189.7±27.2

194.5±25.4

198.9±27.2

200.1±27.2

201.2±27.2

202.8±27.2

203.5±29.2

202.8±27.2

500

209.2±30.2

218.1±20.2

230.9±28.9

238.7±25.1

246.1±30.2

250.2±26.1

252.6±15.3

256.2±26.6

259.1±29.2

259.7±26.1

260.3±28.8

260.12±28.5

1500

196.8±28.2

221.32±28.2

224.3±26.1

226.34±26.2

229.2±26.2

230.2±26.9

234.7±32.2

236.1±27.2

236.7±20.2

238.0±28.2

239.9±32.0

235.0±28.2

Table 3: Effect of oral administration of the aqueous leaf extract of C. ladaniferus on the weight of rats

Table 4

	Liver	Kidneys	Spleen
Control mg/kg	7.20 ±0.7	0.61±0.02	0.51.±0.04
500mg/kg	6.87±0.30	0.62±0.02	0.53±0.05
1500mg/kg	6.67±0.19	0.59±0.03	0.50±0.03

Table 4: Organ weights (g) of the rats in the sub-chronic toxicity studies of the aqueous leaf extract of C. ladaniferus . n = 8, Values are ex- pressed as mean ± SEM, * p >0,05

The values are expressed as mean ± standard deviation of 8 rats in each group. Group I: Control (0 mg / kg); Group II: 500 mg / kg; Group III: 1500 mg / kg.

Table 5

Parameters	Doses (mg/kg)
Parameters	Control	500	1500
Red blood cells (106/µl)	7,70±0,53	7,71 ±0.20*	7,93±0,40 *
White blood cells (103/µl)	9,10± 0,51	9,08± 0,15 *	8,95±0,59 *
Haemoglobin (g/dl)	15,89± 0,85	15,43± 0,69 *	15,66 ±0,62 *
Hématocrit (vol %)	40,87 ±0,45	41,75±0,66 *	41,62±0,8 *
Platelets (104/µl)	70,97± 0,86	70,74±0,8 4*	70,99±0,74 *

Table 5: Study of the haematological parameters of rats after daily oral administration of the aqueous leaf extract of C. ladaniferus for 90 days. n = 8, Values are expressed as mean ± SEM, * p >0,05

Values are expressed as mean ± standard deviation of 8 rats in each group.Group I: Control (0 mg / kg); Group II: 500 mg / kg; Group III: 1500 mg / kg.

Table 6

Parameters	Doses (mg/kg)
Parameters	Control	500	1500
Creatinine (µmol/l)	59±0.91	60,4±0.20 *	61,4±0.55 *
ASAT (U/L)	83,5± 6,52	80,1±6,56 *	84,75±3,19 *
ALAT (U/L)	22,1± 3,87	23,2± 1,28 *	23,5±1,30 *
Total protein (g/l)	82,01±7,41	84,2±7,25 *	82,2±7,48 *

Table 6: Study of the biochemical parameters of rats after daily oral administration of the aqueous leafextractof C. ladaniferus . n = 8, Values are expressed as mean ± SEM, * p >0,05

Values are expressed as mean ± standard deviation of 8 rats in each group. Group I: Control (0 mg / kg); Group II: 500 mg / kg; Group III: 1500 mg / kg.

FIGURE 1

Figure 1: Plot of log dose of aqueous leaf extract of Cistus ladaniferus vs probit mortality values using probit analysis

FIGURE 2

Figure 2: Histological section of male rat kidney (A) and female rat (B) control. Male rat (C) and female rat (D) treated (dose of 500mg / kg). Male rat (E) and female rat (F) treated (dose of 1500mg / kg). Vascular congestion. Hematoxylin Eosin Stain (Average magnification)

FIGURE 3

Figure 3: Histological section of male rat spleen (A) and female rat (B) controls. Male rat (C) and female rat (D) rats treated (500 mg / kg dose) treated male rat (E) and female rat (F) rats (dose of 1500 mg / kg). Eosin hematoxylin staining. (Average magnification)

FIGURE 4

Figure 4: Histological section of male rat liver (A) and female rat (B) controls. Male rat (C) and female rat (D) treated (dose of 500mg / kg). Male rat (E) and female rat (F) treated (dose of 1500mg / kg). Eosin hematoxylin staining. (Average magnification)