Figures at a glance

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FIGURE 1

Figure 1: A) Primary culture (×20) arrows shows non adherent round shape GCs. B) Cell line (×4)

FIGURE 2

Figure 2: The results of flow cytometry analysis. R-1 Gating Zone show stem cells based on cell distribution in cell size (FSC = Forward Scatter) and complexity (SSC = Side Scatter). IgM and IgG are control isotype antibodies conjugated with FITC. Percentage of each markers expressed is shown in the upper right corner of each figure

FIGURE 3

Figure 3: A) Experimental group 1: 7 day cell culture in sperm - inductive medium. Nucleus was still concentrated in the central core of the cell with two appendages from both sides. B) Experimental group 2: 14 day cell culture in sperm - inductive medium. Morphology of the cells had noticeable changes compared to primary cells. The nucleus of a cell was pulled at one end and got a small tail. C) Experimental group 3: 21 day cell culture in sperm - inductive medium. During this week tail had become longer and the shape of the head has changed. D) Experimental group 4: 28 day cell culture in sperm - inductive medium. Tail and head was clear and cells were more similar to sperm morphology. Figures are zoomed after photography.

FIGURE 4

Figure 4: Western blotting analysis: A) GPR125 protein expression analysis B) VASA protein analysis C) DAZL protein analysis. Lane 1 to 4 was loaded with induced cell extract. W indicates for week and the index shows the time of induction. Granulosa cells extract without induction used as negative control and murine testis extract used as positive control

FIGURE 5

Figure 5: Image J software analysis: A) GPR125 B) VASA C) DAZL