
Figure 1: P53 expression and localization changes during cardiac differentiation
(A) Timeline of human in vitro cardiac differentiation of hiPSCs with indicated small molecules used and the days in which samples were collected for RNA, protein, and immunostaining. Developmental stages are indicated by color underneath their corresponding days. (B) RNA was collected from the indicated time points in A and subjected to qRT-PCR and the results compiled into a heatmap. The genes were grouped temporally based on their expression levels with genes identifying pluripotency (orange), cardiac mesoderm (green), cardiac progenitors (blue), and cardiomyocytes (red). These stages are color coded and correlate back to 2A. (n = 4) (C) qRT-PCR of P53 mRNA expression across the cardiac differentiation. (n = 28). (D) Representative western blot and quantification of P53 protein expression during cardiac differentiation (n = 3). (E) Immunostaining for P53 in human iPS colonies (Tra-1-60+), Day 5-cardiac progenitors (NKX2-5 +), and in Day 15 cardiomyocytes (α-actinin +). Scale bars = 20 μm
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