Tables at a glance

Table 1

Figures at a glance

Comments

Figure 1

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Table 1

Primers	Temperature	Sequence
CD44-Forward	60°C	CCTGGCAGCCCCGATTATTT

CD44-Reverse	60°C	AAGGACACACCCAAGCAAGG

PPAR gamma-Forward	50°C	CAGTGTGAATTACAGCAAACC
PPAR gamma-Reverse	50°C	ACAGTGTATCAGTGAAGGAAT

Osteocalcin-Forward	60°C	AGCAAAGGTGCAGCCTTTGT
Osteocalcin-Reverse	60°C	GCGCCTGGGTCTCTTCACT

Collagen 10A1-Forward	60°C	CACGCAGAATCCATCTGAGAATAT
Collagen 10A1-Reverse	60°C	GTTCAGCGTAAAACACTCCATGAA

RPLPO-Forward	60°C	AATCTCCAGGGGCACCATT
RPLPO-Reverse	60°C	CGTTGGCTCCCACTTTGT

Table 1: : Primers for RT PCR expression

FIGURE 1

Figure 1: A: dental tissue explant extruding DPSC; B: a monolayer of spindle-shaped DPSC in tissue culture flask, day 4

FIGURE 2

Figure 2 : : DPSCs expressed mesenchymal stem cell markers such CD44, CD73, CD 105 and CD 90. In addition, the cells did not express the haematopoietic markers such as CD34 and CD45

FIGURE 3

Figure 3: Top panel, tri-lineage differentiation potential of DPSCs. Presence of lipid globules stained with Oil Red O demonstrated in adipogenic-differentiated DPSCs (A). The presence of mineralised matrix in osteoblast differentiated DPSCs stained by Alizarin Red S stain (B). The presence of carboxylated and sulphated proteoglycans in chondrocyte differentiated DPSCs stained by Alcian blue (C). Bottom panel from left, quantification of adipogenic, chondrogenic and osteogenic differentiation

FIGURE 4

Figure 4: RNA expression of undifferentiated DPSCs (A) CD44, Adipogenic differentiated (B) PPAR?, Osteogenic differentiated (C) Osteocalcin (OC), and Chondrogenic differentiated (D) Collagen 10A1 at day 7

FIGURE 5

Figure 5: Exosome concentration and particle size distribution of DPSC exosomes displayed about 70% range from 25 to135 nm, 20% range from 255 to 315 nm and 10% range from 588 to 765 nm

FIGURE 6

Figure 6: Human cytokine array analysis of DPSCs-CM. Densitometric analysis of proteins associated with regenerative potential

FIGURE 7

Figure 7: Left: The dose response of lyophilised DP-CM and AD-CM in scratch wound assay representing significant percentage gap closure that corresponds to the distance migrated by the human dermal fibroblast cells. Right: A representative scratch wound assay images, showing the migration (red arrows) of human dermal fibroblast cells (From Jain, et al. 2019) treated with 5mgs, 10 mgs and 20 mgs DPSCs-CM in comparison to the control at 0 hrs and 24 hrs