Tables at a glance

Table 1

Table 2

Table 3

Figures at a glance

Comments

Figure 1

Comments

Figure 2

Table 1

Reagents	Volume (μL)
2x One-step Buffer	10
One-step Enzyme	1
Assay 1 or 2	4
Internal Control	1
Total volume master mix per reaction	16
Viral RNA	4
Total reaction volume	20

Table 1: Components of the PCR reaction mixture

Table 2

Step	Temperature	Time	Cycles
cDNA syntesis	50°C	30 minutes	1
Pre-denaturation	95°C	15 minutes	1
Denaturation	95°C	15 seconds	45
Annealing & Exstension*	60°C	1 minute	45

*collection of the fluorescence data
Table 2: PCR protocol

Table 3

	July		August		September		October		Total
Age (group)	N	n (%)	N	n (%)	N	n (%)	N	n (%)	N	n (%)
11	21	6 (28.6)	21	3 (14.3)	23	1 (4.3)	42	13 (36.9)	107	23(21.5)
12	11	5 (45.4)	19	5 (26.3)	24	3 (12.5)	46	17 (36.9)	100	30(30.0)
13	8	7 (87.5)	26	5 (19.2)	15	1 (6.7)	38	11 (28.9)	87	24(27.6)
14	10	3 (30.0)	31	1 (3.2)	42	6 (14.3)	40	14 (35)	123	24(19.5)
*(11-14)*	50	*21 (42.0)*	97	*14 (14.4)*	*104*	*11 (10.5)*	*166*	*55 (33.1)*	*417*	*101 (24.2)*
15	17	6 (35.3)	22	6 (27.3)	29	5 (17.2)	72	25 (34.7)	140	42(30.0)
16	19	9 (47.3)	39	9 (23.1)	25	1 (4.0)	72	28 (38.8)	155	47(30.3)
17	21	13 (61.9)	37	7 (18.9)	25	5 (20.0)	73	30 (41.1)	156	55(35.2)
18	18	6 (33.3)	46	13 (28.3)	31	4 (12.9)	56	18 (32.1)	151	41(27.1)
*(15-18)*	75	*34 (45.3)*	*144*	*35 (24.3)*	*110*	*15 (13.6)*	*273*	*101 (40.3)*	*602*	185(30.7)
19	27	15 (55,5)	40	8 (20.0)	31	2 (6.4)	51	24 (47)	149	49(32.8)
20	31	19 (61.3)	41	11 (26.8)	44	4 (9.1)	65	22 (33.8)	181	56(30.9)
21	38	19 (50.0)	62	14 (22.6)	48	5 (10.4)	83	35 (42.2)	231	73(36.3)
22	61	29 (47.5)	65	12 (18.5)	48	7 (14.6)	78	31 (39.7)	252	79(31.3)
23	47	23 (48.9)	106	16 (15.1)	57	9 (15.8)	111	46 (41.4)	321	94(29.3)
24	41	18 (43.9)	98	16 (16.3)	82	18 (21.9)	181	53 (29.3)	402	105(26.1)
*(19-24)*	*245*	*123 (50.2)*	*412*	*77 (18.7)*	*310*	*45 (14.5)*	*569*	*211 (37.1)*	*1536*	*456 (29.7)*
Total:	338	167 (49.4)	653	126 (19.3)	524	71 (13.5)	1 008	367 (36.4)	2523	731 (28.9)

Table 3: Total number of tested and SARS-CoV-2 positive persons during the July-October, 2020

FIGURE 1

Figure 1: SARS- CoV- 2 infected adolescent per observed months and age

FIGURE 2

Figure 2: Prevalence of SARS-CoV-2 infected adolescents per month and age group %- percentage of SARS-CoV-2 infected adolescents