Figure 1: SARS- CoV- 2 infected adolescent per observed months and age
Reagents |
Volume (μL) |
2x One-step Buffer |
10 |
One-step Enzyme |
1 |
Assay 1 or 2 |
4 |
Internal Control |
1 |
Total volume master mix per reaction |
16 |
Viral RNA |
4 |
Total reaction volume |
20 |
Table 1: Components of the PCR reaction mixture
Step |
Temperature |
Time |
Cycles |
cDNA syntesis |
50°C |
30 minutes |
1 |
Pre-denaturation |
95°C |
15 minutes |
1 |
Denaturation |
95°C |
15 seconds |
45 |
Annealing & Exstension* |
60°C |
1 minute |
*collection of the fluorescence data
Table 2: PCR protocol
|
July |
August |
September |
October |
Total |
|||||
Age (group) |
N |
n (%) |
N |
n (%) |
N |
n (%) |
N |
n (%) |
N |
n (%) |
11 |
21 |
6 (28.6) |
21 |
3 (14.3) |
23 |
1 (4.3) |
42 |
13 (36.9) |
107 |
23(21.5) |
12 |
11 |
5 (45.4) |
19 |
5 (26.3) |
24 |
3 (12.5) |
46 |
17 (36.9) |
100 |
30(30.0) |
13 |
8 |
7 (87.5) |
26 |
5 (19.2) |
15 |
1 (6.7) |
38 |
11 (28.9) |
87 |
24(27.6) |
14 |
10 |
3 (30.0) |
31 |
1 (3.2) |
42 |
6 (14.3) |
40 |
14 (35) |
123 |
24(19.5) |
(11-14) |
50 |
21 (42.0) |
97 |
14 (14.4) |
104 |
11 (10.5) |
166 |
55 (33.1) |
417 |
101 (24.2) |
15 |
17 |
6 (35.3) |
22 |
6 (27.3) |
29 |
5 (17.2) |
72 |
25 (34.7) |
140 |
42(30.0) |
16 |
19 |
9 (47.3) |
39 |
9 (23.1) |
25 |
1 (4.0) |
72 |
28 (38.8) |
155 |
47(30.3) |
17 |
21 |
13 (61.9) |
37 |
7 (18.9) |
25 |
5 (20.0) |
73 |
30 (41.1) |
156 |
55(35.2) |
18 |
18 |
6 (33.3) |
46 |
13 (28.3) |
31 |
4 (12.9) |
56 |
18 (32.1) |
151 |
41(27.1) |
(15-18) |
75 |
34 (45.3) |
144 |
35 (24.3) |
110 |
15 (13.6) |
273 |
101 (40.3) |
602 |
185(30.7) |
19 |
27 |
15 (55,5) |
40 |
8 (20.0) |
31 |
2 (6.4) |
51 |
24 (47) |
149 |
49(32.8) |
20 |
31 |
19 (61.3) |
41 |
11 (26.8) |
44 |
4 (9.1) |
65 |
22 (33.8) |
181 |
56(30.9) |
21 |
38 |
19 (50.0) |
62 |
14 (22.6) |
48 |
5 (10.4) |
83 |
35 (42.2) |
231 |
73(36.3) |
22 |
61 |
29 (47.5) |
65 |
12 (18.5) |
48 |
7 (14.6) |
78 |
31 (39.7) |
252 |
79(31.3) |
23 |
47 |
23 (48.9) |
106 |
16 (15.1) |
57 |
9 (15.8) |
111 |
46 (41.4) |
321 |
94(29.3) |
24 |
41 |
18 (43.9) |
98 |
16 (16.3) |
82 |
18 (21.9) |
181 |
53 (29.3) |
402 |
105(26.1) |
(19-24) |
245 |
123 (50.2) |
412 |
77 (18.7) |
310 |
45 (14.5) |
569 |
211 (37.1) |
1536 |
456 (29.7) |
Total: |
338 |
167 (49.4) |
653 |
126 (19.3) |
524 |
71 (13.5) |
1 008 |
367 (36.4) |
2523 |
731 (28.9) |
Table 3: Total number of tested and SARS-CoV-2 positive persons during the July-October, 2020
Figure 1: SARS- CoV- 2 infected adolescent per observed months and age
Figure 2: Prevalence of SARS-CoV-2 infected adolescents per month and age group %- percentage of SARS-CoV-2 infected adolescents
Tables at a glance
Figures at a glance