Tables at a glance

Table 1

Figures at a glance

Comments

Figure 1

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Figure 2

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Figure 3

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Figure 4

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Figure 5

Table 1

Primers	Sequence (5’ to 3’)		Position in VP2	Length (bp)
Fo	GTGATCCAAGATATGCATTTGG		1121- 1142	22
Ro	TAATTTTCTAGGTGCTAGTTGAGA		1749- 1726	24
Fi	CTTTAACCTTCCTGTAACAA		1257-1276	20
Ri	GTTGGTAGCAATACATTATCATC		1298- 1278	23
Primer combinations		Product size (bp)	CPV Variants Detection
Fo + Ro (Set 1)		629	CPV
Fi + Ro (Set 2)		493	CPV 2a
Fo + Ri (Set 3)		178	CPV 2b

Table 1: Primers for Tetra Arms PCR along with their combination

FIGURE 1

Figure 1: Fo primer: 1121-1142 bp in VP2 gene (Red colour), Fi primer 1257-1276 bp in VP2 (underlined); 1276-1278 bp in VP2 gene is the mutated amino acid asparagine (Green colour)

FIGURE 2

Figure 2: Fo, Fi and Ri primer sequence in multiple alignment

FIGURE 3

Figure 3: Ro primer sequence in multiple alignment

FIGURE 4

Figure 4: Conventional PCR for identification of CPV

FIGURE 5

Figure 5: Typing of CPV in TETRA ARMS PCR